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Interferon-induced transmembrane proteins (IFITMs) are restriction factors that inhibit the infectious entry of many enveloped RNA viruses. However, we demonstrated previously that human IFITM2 and IFITM3 are essential host factors facilitating the entry of human coronavirus (HCoV) OC43. In a continuing effort to decipher the molecular mechanism underlying IFITM differential modulation of HCoV entry, we investigated the roles of structural motifs important for IFITM protein posttranslational modifications, intracellular trafficking, and oligomerization in modulating the entry of five HCoVs. We found that three distinct mutations in IFITM1 or IFITM3 converted the host restriction factors to enhance entry driven by the spike proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) and/or Middle East respiratory syndrome coronavirus (MERS-CoV). First, replacement of IFITM3 tyrosine 20 with either alanine or aspartic acid to mimic unphosphorylated or phosphorylated IFITM3 reduced its activity to inhibit the entry of HCoV-NL63 and -229E but enhanced the entry of SARS-CoV and MERS-CoV. Second, replacement of IFITM3 tyrosine 99 with either alanine or aspartic acid reduced its activity to inhibit the entry of HCoV-NL63 and SARS-CoV but promoted the entry of MERS-CoV. Third, deletion of the carboxyl-terminal 12 amino acid residues from IFITM1 enhanced the entry of MERS-CoV and HCoV-OC43. These findings suggest that these residues and structural motifs of IFITM proteins are key determinants for modulating the entry of HCoVs, most likely through interaction with viral and/or host cellular components at the site of viral entry to modulate the fusion of viral envelope and cellular membranes.
IMPORTANCE The differential effects of IFITM proteins on the entry of HCoVs that utilize divergent entry pathways and membrane fusion mechanisms even when using the same receptor make the HCoVs a valuable system for comparative investigation of the molecular mechanisms underlying IFITM restriction or promotion of virus entry into host cells. Identification of three distinct mutations that converted IFITM1 or IFITM3 from inhibitors to enhancers of MERS-CoV or SARS-CoV spike protein-mediated entry revealed key structural motifs or residues determining the biological activities of IFITM proteins. These findings have thus paved the way for further identification of viral and host factors that interact with those structural motifs of IFITM proteins to differentially modulate the infectious entry of HCoVs.
The interferon (IFN)-mediated innate immune response is the first line of defense against virus infections in vertebrates (1, 2). IFNs execute antiviral activity by binding to their cognate receptors on the cell surface to activate a signaling cascade leading to induction of hundreds of IFN-stimulated genes (ISGs) (3, 4). Among the ISGs, those encoding IFN-induced transmembrane (IFITM) proteins, including IFITM1, IFITM2, and IFITM3, are widely expressed and can be induced by all three types of IFNs in many cell types. The IFITMs localize at the cell plasma membrane and endocytic vesicles and restrict the entry of enveloped RNA viruses from nine viral families (5), including some medically important human pathogens, such as influenza A virus (IAV), dengue virus (DENV), West Nile virus, Zika virus, chikungunya virus, Ebola virus (EBOV), Rift Valley fever virus, human immunodeficiency virus (HIV), and hepatitis C virus (3, 6,–15).
Coronaviruses (CoVs) are a large family of enveloped, positive-strand RNA viruses with a broad host range and primarily cause respiratory or enteric diseases, but some of them cause hepatitis, neurological disorders, or cardiomyopathy (16, 17). Human coronaviruses (HCoVs) 229E, OC43, NL63, and HKU1 circulate globally and cause mild upper respiratory tract infections (18) but are occasionally associated with more severe lower respiratory tract diseases in elderly and immunocompromised patients (19). On the other hand, the recently emerging HCoVs, such as severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), cause severe diseases among infected individuals (20, 21). Thus far, several groups have reported that IFITMs inhibited entry of HCoV-229E, HCoV-NL63, SARS-CoV, and MERS-CoV into their host cells with varying efficiency (8, 22, 23).
Concerning the molecular mechanism underlying IFITM restriction of virus entry, the currently favored hypothesis postulates that the existence of IFITM proteins in the endocytic membranes alters either the membrane curvature or fluidity to make the endosomal membrane rigid and less able to fuse with viral envelopes (24, 25,–28). However, several recent findings challenge this hypothesis. First, human IFITM2 and IFITM3 efficiently enhance the infectious entry of HCoV-OC43 via a post-receptor binding/endocytosis mechanism (29). Second, mutation of the SVKS motif in the CD225 domain required for IFITM1 to inhibit HIV-1 entry and for IFITM3 to restrict IAV and dengue virus infection (24, 30) enhances Lloviu virus (LLOV) glycoprotein-mediated entry (see Fig. 11) (31). Third, human IFITM proteins are required for the formation of the human cytomegalovirus virion assembly complex (VAC) and infectious virion secretion (32). The VAC is a perinuclear membrane structure in which the vesicles with endosomal markers occupy the central area and the vesicles with Golgi markers are wrapped around it to form a circle (33). It is possible that IFITMs modulate endosomal trafficking/fusion during VAC formation. All these findings strongly suggest that the IFITM-induced membrane curvature and/or fluidity alterations may not always make the endocytic membranes too “rigid” to fuse but, at least under certain conditions, may facilitate membrane fusion.
In order to further understand the molecular mechanism underlying the differential modulation of HCoV entry by IFITM proteins, we set out to identify structural motifs important for IFITM protein posttranslational modifications, intracellular trafficking, and oligomerization in modulating the entry of five HCoVs. We found that although both SARS-CoV and NL63 use angiotensin-converting enzyme 2 (ACE2) as their entry receptor, IFITMs differentially modulate the entry of the two viruses. We also found that three distinct mutations in IFITM1 or IFITM3 converted the host restriction factors to enhancers of SARS-CoV and/or MERS-CoV entry. These findings imply that restriction or promotion of virus entry by an IFITM may rely on its fine-tuned interaction with viral and host cellular factors via the structural motifs at the site of viral envelope and cellular membrane fusion. Moreover, posttranslation modification of those structural motifs by host cellular factors may alter IFITM interaction with the components of viral entry machinery and consequently change its potency and/or the nature of modulating the entry of a virus.
Viral envelope proteins and cellular receptors are the major players in virus entry into their host cells. It is conceivable that IFITM interaction with the viral envelope and/or cellular receptors may play an important role in restriction of virus entry. ACE2, as the common receptor for both SARS-CoV and NL63, provides a unique opportunity to investigate the role of viral receptors in IFITM modulation of HCoV entry (34). Accordingly, we examined the effects of three human IFITM proteins on the entry of four HCoVs, with Lassa fever virus (LASV) and IAV as negative and positive controls, in HEK293 cells and the Huh7.5 hepatoma cell line, which express detectable and undetectable basal levels of IFITMs, respectively (29) (Fig. 1). As anticipated, expression of any of the three IFITMs in either HEK293 or Huh7.5 cells did not inhibit infection by lentiviral particles pseudotyped with the envelope proteins of Lassa fever virus (LASVpp) (Fig. 1B and andC).C). However, all three IFITMs significantly inhibited the infection by lentiviral particles pseudotyped with IAV hemagglutinin 1 (H1) and neuraminidase 1 (N1) (IAVpp), Spike protein (S) of HCoV-229E (229Epp), HCoV-NL63 (NL63pp), SARS-CoV (SARSpp), and MERS-CoV (MERSpp) in both HEK293 (Fig. 1B) and Huh7.5 (Fig. 1C) cells. Comparing the extent of IFITM inhibition in the two cell lines (Fig. 1B and andC),C), IFITM2 and IFITM3 inhibition of IAVpp infection was 4- and 20-fold more potent, respectively, in HEK293 cells than in Huh7.5 cells. On the other hand, IFITM2 and IFITM3 more efficiently inhibited entry of all four HCoVpp, particularly MERSpp and 229Epp, in Huh7.5 cells. The steady-state levels of IFITM1 were lower than those of IFITM2 and IFITM3 in both HEK293 and Huh7.5 cells, which may, at least in part, explain its lower ability to inhibit infection by all the tested pseudoviruses except 229Epp. Interestingly, IFITM1 more potently inhibited 229Epp infection than IFITM2/3 in HEK293 cells but was less effective in Huh7.5 cells. While the viral envelope proteins are obviously the primary determinants of the potency of IFITMs to restrict virus entry, the more potent inhibition by all three IFITMs of infection by NL63pp than by SARSpp suggests that host cellular factors other than viral receptors have a strong impact on antiviral activity of IFITMs.
We next aimed at identifying IFITM structural motifs that control the modulation of HCoV entry. IFITM proteins contain a conserved CD225 domain flanked by sequence-divergent N- and C-terminal variable regions (5). The N-terminal 21 amino acid residues unique to IFITM2 or -3 have been demonstrated to be important for IFITM3 to inhibit IAV infection in cultured cells and in vivo in humans (35,–38). It has been shown recently that the N-terminal region of IFITM3 contains a 20YEML23 tetrapeptide that is consistent with the canonical YXXΦ endocytic sorting signal (where X can be any amino acid and Φ denotes Val, Leu, or Ile) (39,–41). Furthermore, Y20 can be phosphorylated by the tyrosine kinase Fyn, which regulates IFITM3 trafficking and metabolism (42). We therefore investigated how the phosphorylation of IFITM3 at Y20 may regulate its function of modulating HCoV entry. The results showed that, compared to wild-type IFITM3, replacement of Y20 with alanine (A) and aspartic acid (D) or glutamic acid (E) to mimic the nonphosphorylated (Y20A) or phosphorylated (Y20E or Y20D) IFITM3, respectively, did not alter the steady-state levels of expression (Fig. 2A and andF)F) and activity to enhance the entry of HCoV-OC43 in both HEK293 and Huh7.5 cells (Fig. 2B and andG).G). However, the mutant IFITM3 proteins showed significantly reduced activity to inhibit NL63pp and 229Epp infection (Fig. 2C, ,D,D, ,H,H, and andI).I). On the other hand, mutant IFITM3 proteins enhanced infection by SARSpp and MERSpp in both cell lines (Fig. 2C, ,E,E, ,H,H, and andJ).J). Consistent with previous reports (39, 40), wild-type IFITM3 accumulated in the perinuclear region and primarily colocalized with Rab9, a later endosome marker (43) (Fig. 3A). In contrast, IFITM3 proteins bearing a Y20A or Y20D mutation primarily accumulated in the regions close to the plasma membrane (Fig. 3B and andC).C). These results indicate that Y20 is critical for endocytic sorting, which is regulated by tyrosine phosphorylation.
In order to investigate whether the enhanced infection by SARSpp and MERSpp by the mutant IFITM3 proteins is due to the induction of membrane fusion on the plasma membrane, we examined the effect of endosomal pH on HCoVpp infection of Huh7.5 cells expressing wild-type or Y20A IFITM3 (Fig. 4). As shown in Fig. 4A, among the five tested HCoVpp, MERSpp and NL63pp infections were less sensitive to NH4Cl treatment that elevated endosomal pH, suggesting that membrane fusion for the two viruses may occur in early endosomal compartments. Interestingly, IFITM3 Y20A-enhanced SARSpp and MERSpp infections were efficiently inhibited by NH4Cl treatment in a concentration-dependent manner (Fig. 4B and andC).C). The results thus suggest that although Y20 mutant IFITM3 proteins primarily accumulate in the plasma membrane regions, enhanced infection by SARSpp and MERSpp still occurs in low-pH endosomal compartments.
In addition to Y20, Y99 had been shown by mass spectrometry analysis to be phosphorylated in cells and to play a role in restricting the infectious entry of IAV, but not dengue virus (24). Therefore, we performed phosphomimetic analysis on this amino acid residue. As shown in Fig. 5A, the Y99A or Y99D mutant IFITM3 was expressed to a level similar to that of wild-type IFITM3. Compared with wild-type IFITM3, Y99A or Y99D mutants had slightly increased activity to enhance OC43pp infection but significantly reduced activity to inhibit infection by SARSpp, NL63pp, IAVpp, and vesicular stomatitis virus (VSVpp) (Fig. 5B to toE).E). Intriguingly, both Y99A and Y99D IFITM3 enhanced MERSpp infection by approximately 10-fold (Fig. 5F). The results imply that Y99 plays a critical role in IFITM3 modulation of the entry of different HCoVs.
In addition to phosphorylation, the function of IFITM proteins is regulated by cysteine palmitoylation (44, 45), ubiquitination (46, 47), and homo- and hetero-oligomerization (24, 29). To investigate the roles of these posttranslational modifications in IFITM3 inhibition of HCoV entry, HEK293 cell lines inducibly expressing wild-type or mutant IFITM3 proteins bearing mutations that preclude cysteine palmitoylation or ubiquitination were established. Specifically, the conserved cysteine residues C71 and C72 or one additional cysteine, C105, which are critical for IFITM3 palmitoylation, were replaced with alanine to yield two mutants, IFITM3/2CA and IFITM3/3CA, respectively. As shown in Fig. 6A and andB,B, the mutations had minimal impacts on protein expression but completely abolished activity to restrict infection by all five pseudoviruses sensitive to IFITM3. However, IFITM3/4KA, which were generated by replacement of K24, K83, K88, and K104 with alanines, accumulated at a significantly reduced level in cells and failed to inhibit infection by all the pseudoviruses examined. Moreover, IFITM3 containing F75A and F78A mutations (IFITM3/2FA), which disrupt its oligomerization (29), completely lost the ability to inhibit infection by SARSpp, 229Epp, MERSpp, and IAVpp but could still significantly inhibit infection by NL63pp, despite reduced activity (Fig. 6B). The results thus suggest that, unlike other viruses (24, 29), suppression of NL63 spike protein-triggered membrane fusion does not absolutely require the oligomerization of IFITM3.
We showed previously that sequential truncation of the C-terminal 18 amino acid residues from IFITM1 did not apparently affect its activity to inhibit infection by IAVpp but converted the antiviral protein into an increasingly potent enhancer of OC43pp infection (29). In our efforts to further dissect the role of the C-terminal domain (CTD) in IFITM1 modulation of HCoV entry, we found that deletion of the C-terminal 3, 6, or 9 amino acids did not apparently affect the activity of IFITM1 to inhibit infection by SARSpp, but further deletion of the C-terminal 12, 15, or 18 amino acids significantly compromised or abolished the ability of IFITM1 to inhibit infection by SARSpp (Fig. 7B). In contrast, deletion of the C-terminal 3, 6, and 9 amino acids enhanced the activity of IFITM1 to inhibit infection by NL63pp by 5-, 10-, and 3-fold, respectively. However, further truncation of the C-terminal 12, 15, or 18 amino acids abolished the enhanced inhibitory effect on NL63pp infection (Fig. 7B). Interestingly, sequential truncation of the CTD gradually attenuated and ultimately abolished the activity of IFITM1 to inhibit 229Epp infection (Fig. 7C). On the other hand, sequential truncation of the C-terminal 12 amino acids gradually increased its activity to enhance MERSpp infection, but further deletion of the C-terminal 15 or 18 amino acids reduced its activity to enhance MERSpp infection (Fig. 7D).
To rule out the potential interference of endogenous IFITM proteins with mutant IFITM1 in HEK 293 cells (29), we further confirmed the observation in Huh7.5 cells that expressed undetectable endogenous IFITM proteins. Four Huh7.5 cell lines were established by transduction with an empty retroviral vector or a retroviral vector expressing wild-type IFITM1, IFITM1/TC6, or IFITM1/TC18 protein. Consistent with the observations made in HEK293 cells, deletion of the C-terminal 18, but not 6, amino acids significantly compromised IFITM1's ability to suppress SARSpp infection, and deletion of the C-terminal 6, but not 18, amino acids significantly enhanced activity to inhibit NL63pp infection (Fig. 8B). In agreement with the results obtained with NL63pp infection, we also observed that deletion of the C-terminal 6, but not 18, amino acids significantly increased the ability of IFITM1 to inhibit infection by HCoV-NL63, as judged by significant reduction in the percentages of infected cells and intracellular viral RNA (Fig. 8C and andDD).
Taken together, the results suggest that two partially overlapping functional motifs exist in the CTD of IFITM1. While its C-terminal 9 to 12 amino acid residues contain a motif that downregulates antiviral activity against HCoV-NL63 and suppresses activity enhancing MERS-CoV infection, the motif located in the N-terminal 9 amino acid residues of the CTD is important for IFITM1 to suppress SARS-CoV and HCoV-229E entry.
To investigate the molecular mechanisms underlying the differential modulation of HCoV entry by the CTD, we examined the subcellular localization of wild-type and mutant IFITM1 proteins. As shown in Fig. 9, similar to wild-type IFITM1, IFITM1/TC6 and IFITM1/TC18 primarily accumulate in regions close to or at the plasma membrane. However, modestly increased intracellular localization of both IFITM1/TC6 and IFITM1/TC18 is evident. Specifically, IFITM1/TC6 tends to more frequently colocalize with Rab5 and Rab9, whereas IFITM1/TC18 is more frequently colocalized with EEA1, an early endosomal marker (48), and Rab9, a later endosomal marker. Moreover, as shown in Fig. 10, compared to wild-type IFITM1, IFITM1/TC6, but not IFITM1/C18, demonstrated reduced mono- and diubiquitination. As anticipated, replacement of four lysine residues in IFITM3 (IFITM3/4KA) completely abolished ubiquitination. The results thus indicate that the CTD of IFITM1 contains a structural motif(s) that regulates its subcellular trafficking and ubiquitination, which may consequentially affect its activity to modulate the entry of HCoVs.
In spite of the relatively broad spectrum of antiviral activities, IFITM proteins do not restrict infection by MLV, Sendai virus, and several members of the family Arenaviridae, as well as all the DNA viruses tested thus far (6, 8, 49, 50). The molecular determinants that control the viral specificity and potency of IFITMs remain to be fully understood. Because viral envelope proteins and cellular receptors are the two major players in viral membrane fusion, it is plausible to consider that modulating the interaction between viral envelope proteins and cellular receptors might be the key mechanism of IFITM restriction of virus entry. Indeed, it was reported recently that the IFITM sensitivity of HIV-1 strains is determined by the coreceptor usage of viral envelope glycoproteins (51, 52). However, the difference in the potency and the requirement for IFITM oligomerization in inhibition of SARSpp and NL63pp infection (Fig. 1 and and6)6) and, particularly, the results showing that IFITM3/Y20 phosphomimetic mutations enhanced only infection by SARSpp, but not by NL63pp (Fig. 2), strongly suggest that IFITM interaction with viral and host cellular factors, other than viral receptors, such as ACE2, plays a critical role in IFITM modulation of virus entry. Furthermore, our findings that IFITM2 and IFITM3 promoted HCoV-OC43 infection and that three distinct mutations converted IFITM1 and IFITM3 from inhibitors to enhancers of SARS-CoV and/or MERS-CoV spike protein-mediated entry challenge the “rigid-membrane” hypothesis and suggest that IFITM proteins may also promote membrane fusion, under select conditions, to facilitate virus entry.
Based on these new findings, we hypothesize that, depending on the fine-tuned interaction with the entry machinery of a given virus, which consists of viral envelope components, as well as viral receptors and other host entry factors at the site of membrane fusion, IFITM proteins can either promote or arrest the fusion between viral envelope and endosomal membranes (29). It is possible that the three structural motifs identified here mediate interactions with key host factors to determine either to arrest or to enhance membrane fusion. Along these lines, recent studies revealed that zinc metallopeptidase STE24 forms complexes with IFITM proteins and is required for IFITMs to inhibit the entry of many different viruses (53). In addition, the sensitivity of IAVs to IFITM3 appears to depend on the pH value at which the viral hemagglutinin (HA) undergoes a conformational transition and mediates membrane fusion (54). More interestingly, IFITM expression promotes the uptake of avian sarcoma leukosis virus (ASLV) and the acidification of endosomal compartments, resulting in accelerated membrane fusion when driven by the glycosylphosphatidylinositol-anchored, but not by the transmembrane, isoform of the ASLV receptor (55). These recent findings clearly highlight the fact that multiple viral and host cellular components regulate IFITM activity in the fusion of viral envelope and endosomal membranes.
Our new hypothesis predicts that in order to modulate virus entry, IFITM proteins ought to be at the site of viral membrane fusion (26,–28). Indeed, previous studies demonstrated in a variety of virus infection systems that localization in the subcellular compartment where a virus enters the cytoplasm is important for the IFITM protein to inhibit its infectious entry (5). For instance, disruption of the canonical endocytic signal in IFITM3 by Y20A/E/D mutations resulted in its plasma membrane accumulation (Fig. 11). As a consequence, the mutant IFITM3 demonstrated reduced antiviral activity against IAV, which enters cells by fusion with the lysosomal membrane (39,–41), but enhanced the activity to restrict infection by parainfluenza virus 3, a virus that enters cells by fusion with the plasma membrane (56). However, the subcellular localization of IFITM proteins is not always strictly correlated with antiviral activity. For example, while IFITM3 Y99A mutation does not apparently alter subcellular localization, the mutation significantly compromises the antiviral activity of IFITM3 against IAV, but not dengue virus (24). In this study, we further demonstrated that although Y20A mutant IFITM3 predominantly accumulated in the plasma membrane region, its enhancement of infection by SARSpp, MERSpp, and OC43pp was still low pH dependent (Fig. 4) (29), suggesting that the enhanced entry of the viruses still occurs in low-pH intracellular endosomal compartments. The apparent contradiction between the subcellular localization of the mutant IFITM3 proteins and the site of membrane fusion implies that a small fraction of the mutant IFITM3 might still traffic to the sites where the viral spike protein-induced membrane fusion occurs. In addition, phosphomimetic analyses suggest that Y20 or Y99 tyrosine phosphorylation regulates the metabolism, trafficking, and biological function of IFITM3.
In addition to tyrosine phosphorylation, IFITM3 can also be posttranslationally modified at more than 8 different amino acid residues with at least three different types of modifications: palmitoylation, ubiquitination, and methylation (42, 44,–47, 57). Our mutagenesis studies showed that both palmitoylation and ubiquitination are absolutely required for IFITM3 to modulate the entry of all the tested HCoVs (Fig. 6). Moreover, oligomerization of IFITM proteins has been demonstrated to be essential for their restriction of IAV and dengue virus infection, as well as enhancement of HCoV-OC43 infection (24, 29). Interestingly, in hetero-oligomerization between IFITM1 and IFITM3, which inhibit and enhance HCoV-OC43 infection, respectively, the two proteins antagonize each other's functions (29). In this study, we further revealed that IFITM3 bearing F75A and F78A mutations, which disrupt its oligomerization, completely lost its ability to inhibit infection by SARSpp, 229Epp, and MERSpp but still partially inhibited NL63pp infection (Fig. 6 and and11).11). While the results reinforce the notion that oligomerization is important for IFITMs to modulate the entry of many viruses, NL63 appears to be an exception.
It was reported previously that the CTD of IFITM1, illustrated in Fig. 11, plays an important role in modulating the entry of HCoV-OC43 (29), HIV-1 (58), Jaagsiekte sheep retrovirus, and 10A1 amphotropic murine leukemia virus (59). In this study, we showed that the CTD of IFITM1 plays distinct roles in modulating the entry of different HCoVs. It appears that the CTD contains two overlapping functional motifs. While the C-terminal 9 and 12 amino acid residues negatively regulate IFITM restriction of HCoV-NL63 entry and enhancement of MERS-COV infection, the motif located in the N-terminal 9 amino acid residues of the CTD is important for IFITM1 to suppress SARS-CoV and HCoV-229E entry. In a search for the underlying mechanism, a study identified a dibasic 122KRXX125 motif at the C terminus of IFITM1 that regulates IFITM1 intracellular trafficking with reduced localization in LAMP1-positive lysosomes but increased levels in CD63-positive multivesicular bodies (59). IFITM1 binds to cellular adaptor protein complex 3 (AP-3), an association that is lost when the dibasic motif is altered (59). However, we found that partial or complete deletion of the CTD does not dramatically alter its subcellular distribution (Fig. 9). Instead, deletion of 6, but not 18, amino acid residues from the C terminus reduced IFITM1 ubiquitination (Fig. 10). The results collectively indicate that the CTD is a key regulator of IFITM function. However, its effects on the entry of different viruses imply versatile functions of the CTD. Further investigation into the structure, posttranslational modification, and membrane topology of the CTD, as well as identification of the cellular and/or viral proteins interacting with the CTD, will shed light on the mechanism by which the CTD regulates the function of IFITM1.
In summary, we demonstrated in this study that, in addition to viral envelope proteins and cellular receptors, IFITM protein oligomerization, posttranslational modification, and intracellular trafficking, which can be regulated by host cellular pathobiological cues, play critical roles in determining the extent and nature of IFITM modulation of the entry of HCoVs. More importantly, identification of the three structural motifs that reverse the functions of IFITM1 and IFITM3 on virus entry paves the way for uncovering viral and host factors that interact with those structural motifs to differentially modulate the infectious entry of HCoVs and other viruses (53).
LLC-MK2 cells were cultured in minimal essential medium (MEM), which was prepared by mixing Hanks MEM (Invitrogen; catalog no. 11575-032) and Earle's MEM (Invitrogen; catalog no. 11095-080) in a 2:1 ratio and supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen). Huh7.5 cells were cultured with Dulbecco's modified Eagle's medium (DMEM) (Corning) supplemented with 10% FBS, 1× nonessential amino acids (Invitrogen), and 2 mM l-glutamine (Invitrogen). The human colon cancer cell line HCT-8 was grown in RPMI 1640 medium (ATCC; catalog no. 30-2001) supplemented with 10% FBS. GP2-293 and Lenti-X 293T cell lines were purchased from Clontech and cultured in DMEM supplemented with 10% FBS and 1 mM sodium pyruvate (Invitrogen). FLP-IN T Rex 293 cells were purchased from Invitrogen and maintained in DMEM supplemented with 10% FBS, 10 μg/ml blasticidin (Invitrogen), and 100 μg/ml zeocin (Invivogen) (60). HCoV-NL63 was purchased from ATCC through BEI Resource (catalog no. NR470) and amplified in LLC-MK2 cells. Virus titers were determined by plaque assay (29). Monoclonal antibody against FLAG tag (anti-FLAG M2), rabbit anti-FLAG polyclonal antibody, and β-actin antibody were purchased from Sigma (catalog no. F1804, F7425, and A2228, respectively). A mouse monoclonal antibody against HCoV-NL63 nucleocapsid (N) protein was purchased from Ingenansa, Spain (catalog no. M.30.HCo.I2D4).
pcDNA5/FRT-derived plasmids expressing chloramphenicol acetyltransferase (CAT) and N-terminally FLAG-tagged human IFITM1, IFITM2, and IFITM3, as well as C-terminally truncated IFITM1 mutants, were reported previously (7, 29, 60, 61). Plasmids expressing N-terminally FLAG-tagged mutant IFITM3 proteins with point mutations were constructed by overlap extension PCR (60). All the resulting plasmids were sequenced to verify the desired mutation(s). N-terminally FLAG-tagged human IFITM1, IFITM2, and IFITM3 and their mutants were also cloned into the pQCXIP vector (Clontech) between the NotI and BamHI sites (29). Plasmids expressing HCoV-OC43 S and HE proteins, VSV G protein, H1N1 IAV (A/WSN/33) HA and neuraminidase (NA), Ebola virus (EBOV) GP protein, LASV GP protein, murine leukemia virus (MLV) envelope protein, and HCoV-NL63 and SARS-CoV spike proteins were described previously (62, 63). The MERS-CoV spike gene (GenBank accession number AFS88936) was synthesized by GeneScript, cloned into the pCAGGS vector, and confirmed by DNA sequence analyses. Plasmid pNL4-3.Luc.R−E− was obtained through the NIH AIDS Research and Reference Reagent Program (64, 65). ACE2, aminopeptidase N (APN), and dipeptidyl peptidase 4 (DPP4) cDNA clones were obtained from Origene and cloned into a pcDNA3 vector (Invitrogen) to yield plasmids pcDNA3/ACE2, pcDNA3/APN, and pcDNA3/DDP4, respectively (66).
The various viral envelope protein-pseudotyped lentiviruses bearing luciferase reporter genes, as well as VSV G protein-pseudotyped retroviruses expressing wild-type and mutant IFITM proteins, were packaged as reported previously (66, 67). Each pseudotype was titrated by infection of cells with a serial dilution of pseudotype preparations. The modulation by IFITM of the transduction of a given pseudotype was determined with a titrated amount of pseudotypes that yielded a luciferase signal between 10,000 and 1,000,000 light units per well of 96-well plates. For a given pseudotype, the input of pseudoviral particles was consistent across all the experiments.
Huh7.5 cells in each well of 6-well plates were incubated with 2 ml of Opti-MEM medium containing pseudotyped retroviruses and centrifuged at 20°C for 30 min at 4,000 × g. Forty-eight hours postransduction, the cells were cultured with medium containing 2 μg/ml of puromycin for 2 weeks. The antibiotic-resistant cells were pooled and expanded into cell lines stably expressing human wild-type or mutant IFITM proteins (66). FLP-IN T Rex 293-derived cell lines expressing mutant IFITM proteins in a tetracycline (Tet)-inducible manner were established as previously described (7, 60).
Cell monolayers were washed once with phosphate-buffered saline (PBS) and lysed with 1× Laemmli buffer. An aliquot of cell lysate was separated on a NuPAGE Novex 4 to 12% Bis-Tris gel (Invitrogen) and electrophoretically transferred onto a nitrocellulose membrane (Invitrogen). The membranes were blocked with PBS containing 5% nonfat dry milk and probed with the desired antibody. The bound antibodies were visualized with IRDye secondary antibodies and by imaging with the Li-Cor Odyssey system (Li-Cor Biotechnology).
Total cellular RNA was extracted using TRIzol reagent (Invitrogen) and reverse transcribed using SuperScript III (Invitrogen). Quantitative PCR (qPCR) was performed as previously described on a LightCycler 480II (Roche) with a modified forward primer (5′-AAA CCT CGT TGG AAG CGT GTTC-3′) and reverse primer (5′-CTG TGG AAA ACC TTT GGC ATC-3′) under the following conditions: denaturing at 95°C for 10 min and 45 cycles of amplification (15 s at 95°C and 1 min at 60°C). The PCR amplified a fragment of the HCoV-NL63 N gene (GenBank Gene ID 2943504).
T Rex 293-derived IFITM-expressing cell lines were seeded into 96-well plates with black walls and clear bottoms and transfected with plasmids encoding ACE2, APN, or DPP4 to express viral receptors. For Huh7.5-derived IFITM-expressing cell lines, cells were seeded into black-wall 96-well plates. The cells were infected at 24 h posttransfection by seeding with the desired pseudotyped lentiviral particles for 2 h and then replenished with fresh medium. Two days postinfection, the medium was removed and the cells were lysed with 20 μl/well of cell lysis buffer (Promega) for 15 min, followed by adding 50 μl/well of luciferase substrate (Promega). The firefly luciferase activities were measured by luminometry in a TopCounter (PerkinElmer) (66).
To visualize HCoV-NL63-infected cells, the infected cultures were fixed with 4% paraformaldehyde for 20 min. After permeabilization with 0.1% Triton X-100, the cells were stained with a monoclonal antibody recognizing HCoV-NL63 N protein (Ingenansa, Spain; catalog no. M.30.HCo.I2D4). Bound antibodies were visualized by using Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam; catalog no. ab150113). Cell nuclei were counterstained with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI).
For determination of mutant IFITM1 or IFITM3 subcellular localization, T Rex 293-derived cell lines inducibly expressing mutant IFITM proteins were fixed and permeabilized as described above. The cells were then stained with anti-FLAG monoclonal antibody, together with a rabbit-derived polyclonal antibody against EEA1 (Cell Signaling; catalog no. 2411), Rab5 (Cell Signaling; catalog no. 2143), or Rab9 (Cell Signaling; catalog no. D52G8). The bound antibodies were visualized using Alexa Fluor 594-labeled goat anti-mouse IgG (red) and Alex Fluor 488-labeled goat anti-rabbit IgG (green). Cell nuclei were counterstained with DAPI. Images were sequentially acquired on an FV1000 confocal microscope (Olympus) with a PlanApoN 60×/1.42 numerical aperture objective (Olympus). The pinhole size was adjusted to 1 Airy unit. The optimal diffraction-limited spatial resolution was obtained using a pixel size of 82 nm/pixel. DAPI was excited at 405 nm, and its fluorescence emission was collected between 430 nm and 470 nm. Alexa Fluor 488 was excited at 488 nm, and its fluorescence emission was collected between 505 and 525 nm. Alexa Fluor 594 was excited at 543 nm, and its fluorescence emission was collected between 560 and 660 nm. Negative controls were performed to make sure that there were no significant spectral bleedthrough artifacts between channels.
Confluent LLC-MK2 cells cultured in 96-well plates were infected with 200 μl of Opti-MEM containing serial 10-fold dilutions of viral stock for 2 h at 33°C. The cells were then cultured at 33°C with MEM containing 2.5% FBS for 6 days. The cells from each well with cytopathic effect (CPE) were visualized and counted under a microscope. The 50% tissue culture infective dose (TCID50) of virus stock was measured and converted to PFU per milliliter (68).
To detect ubiquitination of IFITM protein and its mutants, immunoprecipitation (IP)-Western blotting was performed as reported previously (29, 44). Briefly, 293T cells were transfected with plasmids expressing FLAG-tagged IFITM1, IFITM3, and their mutants. The cells were lysed at 48 h posttransfection with lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, and protease inhibitors (Roche) and immunoprecipitated with a monoclonal antibody against FLAG epitope, followed by incubation with protein A/G-agarose beads (Pierce) and washing with Tris-buffered saline (TBS). The immunocomplexes were resolved in a NuPAGE Novex 4 to 12% Bis-Tris gel in morpholineethanesulfonic acid (MES) buffer (Invitrogen) and transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was probed with rabbit polyclonal antibody against FLAG epitope (Sigma; catalog no. F7425) or anti-ubiquitin rabbit polyclonal antibody (Proteintech; catalog no. 10201-2-AP). The bound antibodies were visualized with IRDye secondary antibodies and imaged with a Li-Cor Odyssey system (Li-Cor Biotechnology).
This work was supported by grants from the U.S. National Institutes of Health (AI113267), the National Natural Science Foundation of China (81571976), and the Commonwealth of Pennsylvania through the Hepatitis B Foundation.